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Recombinant Human ENDOG 293 Cell Lysate

Cat.No. : ENDOG-6602HCL
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  • Gene Information
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Description : Antigen standard for endonuclease G (ENDOG), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ENDOG endonuclease G [ Homo sapiens ]
Official Symbol : ENDOG
Synonyms : ENDOG; endonuclease G; endonuclease G, mitochondrial; endo G; mitochondrial endonuclease G; FLJ27463;
Gene ID : 2021
mRNA Refseq : NM_004435
Protein Refseq : NP_004426
MIM : 600440
UniProt ID : Q14249
Chromosome Location : 9q34.1
Pathway : Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem;
Function : endonuclease activity; hydrolase activity; metal ion binding; nucleic acid binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the structure ENDOG? 09/17/2022

The structure of ENDOG includes multiple functional domains, including a hydrophobic transmembrane domain at the N-terminus, an uncoupling domain at the intermediate, and a proton pump domain at the C-terminus.

How is ENDOG involved in the uncoupling of the inner mitochondrial membrane? 01/25/2022

It catalyzes the electron transfer of NADH+H⁺ to the outside mitochondria via the uncoupling domain, while converting the proton electrochemical gradient of the inner mitochondrial membrane into heat energy.

How it affects energy metabolism and nutrient metabolism ENDOG? 11/14/2021

ENDOG regulates mitochondrial energy metabolism and nutrient metabolism processes through uncoupling and proton pump action. For example, it can transfer electrons of NADH+H⁺ to the outside of mitochondria while converting the proton electrochemical gradient of the inner mitochondrial membrane into heat energy.

Is it related to other ENDOG proteins? 09/03/2021

ENDOG HAS A VARIETY OF ASSOCIATIONS WITH OTHER PROTEINS. For example, it may interact with other proteins on the inner mitochondrial membrane to co-participate in mitochondrial function, and it may also interact with proteins in signal transduction pathways to participate in cell signaling.

What are the differences in the expression levels of ENDOG in different tissues and cells? 05/31/2021

There are differences in the expression levels of ENDOG in different tissues and cells. For example, it is expressed higher in muscle tissue and lower in brain tissue. This difference may be related to the energy requirements and metabolic characteristics of different tissues and cells.

What conditions might be indicated by abnormal ENDOG levels? 04/29/2021

Abnormal ENDOG levels may indicate a variety of diseases, especially tumors, neurodegenerative diseases, etc. For example, in tumors such as lung, breast, and colon cancer, expression levels of ENDOG may be abnormally elevated.

Customer Reviews (3)

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Reviews
04/24/2022

    This protein synthesis process is efficient and reliable, reducing experimental time and cost.

    12/27/2021

      ENDOG is easy to purify and manipulate, which improves the experimental efficiency.

      11/22/2021

        The sensitivity and accuracy of the experiment were significantly improved by using this protein.

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