Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human EHMT2 293 Cell Lysate

Cat.No. : EHMT2-6684HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a-SPI is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : EHMT2 euchromatic histone-lysine N-methyltransferase 2 [ Homo sapiens ]
Official Symbol : EHMT2
Synonyms : EHMT2; euchromatic histone-lysine N-methyltransferase 2; BAT8, C6orf30, chromosome 6 open reading frame 30 , HLA B associated transcript 8; histone-lysine N-methyltransferase EHMT2; Em:AF134726.3; G9A; KMT1C; NG36/G9a; protein G9a; H3-K9-HMTase 3; G9A histone methyltransferase; HLA-B associated transcript 8; HLA-B-associated transcript 8; lysine N-methyltransferase 1C; ankyrin repeat-containing protein; histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; BAT8; GAT8; NG36; C6orf30; FLJ35547; DKFZp686H08213;
Gene ID : 10919
mRNA Refseq : NM_006709
Protein Refseq : NP_006700
MIM : 604599
UniProt ID : Q96KQ7
Chromosome Location : 6p21.3
Pathway : Gene Expression, organism-specific biosystem; Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; RNA Polymerase I Promoter Clearance, organism-specific biosystem; RNA Polymerase I Transcription, organism-specific biosystem; RNA Polymerase I Transcription Initiation, organism-specific biosystem; RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription, organism-specific biosystem;
Function : histone methyltransferase activity (H3-K27 specific); histone methyltransferase activity (H3-K9 specific); metal ion binding; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity; transferase activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
What are the consequences of dysregulated EHMT2 expression in cancer cells? 03/08/2022

Aberrant EHMT2 expression is associated with cancer progression, contributing to altered gene expression patterns and epigenetic changes in cancer cells.

How is the activity of EHMT2 regulated at the molecular level? 10/31/2021

EHMT2 activity can be regulated by post-translational modifications, protein-protein interactions, and its association with cofactors and other chromatin-modifying enzymes.

Can EHMT2-mediated H3K9 methylation affect the recruitment of chromatin remodeling complexes? 12/25/2019

Yes, EHMT2-mediated H3K9 methylation can influence the recruitment of chromatin remodeling complexes, impacting the overall chromatin structure and gene expression.

What is the role of EHMT2 in X-chromosome inactivation? 09/20/2018

EHMT2 is involved in X-chromosome inactivation by contributing to the establishment of repressive chromatin marks on one of the X chromosomes in female cells.

How is the expression of the EHMT2 gene regulated in cells? 07/09/2018

The expression of EHMT2 is regulated by various factors, including cellular signaling pathways and epigenetic modifications, contributing to its dynamic role in different cellular contexts.

Customer Reviews (3)

Write a review
Reviews
10/22/2021

    The quality of this recombined protein is unmatched, providing robust and consistent results in our assays.

    02/29/2020

      Timely delivery is crucial for our lab operations, meeting our expectations.

      06/05/2019

        Excellent customer service—responsive and knowledgeable staff helped us choose the most suitable recombined protein for our experiments.

        Ask a Question for All EHMT2 Products

        Required fields are marked with *

        My Review for All EHMT2 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends