"DYNLL2" Related Products

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Recombinant Human DYNLL2 293 Cell Lysate

Cat.No.: DYNLL2-6756HCL
Description: Antigen standard for dynein, light chain, LC8-type 2 (DYNLL2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DYNLL2 dynein, light chain, LC8-type 2 [ Homo sapiens ]
Official Symbol: DYNLL2
Synonyms: DYNLL2; dynein, light chain, LC8-type 2; dynein light chain 2, cytoplasmic; Dlc2; DNCL1B; MGC17810; radial spoke 22 homolog (Chlamydomonas); RSPH22; DLC8b; radial spoke 22 homolog; 8 kDa dynein light chain b;
Gene ID: 140735
mRNA Refseq: NM_080677
Protein Refseq: NP_542408
MIM: 608942
UniProt ID: Q96FJ2
Chromosome Location: 17q23.2
Pathway: Activation of BH3-only proteins, organism-specific biosystem; Activation of BMF and translocation to mitochondria, organism-specific biosystem; Apoptosis, organism-specific biosystem; Intrinsic Pathway for Apoptosis, organism-specific biosystem; Vasopressin-regulated water reabsorption, organism-specific biosystem; Vasopressin-regulated water reabsorption, conserved biosystem;
Function: cytoskeletal protein binding; motor activity;

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