"DPPA2" Related Products

Recombinant Human DPPA2 293 Cell Lysate

Cat.No.: DPPA2-6827HCL
Description: Antigen standard for developmental pluripotency associated 2 (DPPA2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DPPA2 developmental pluripotency associated 2 [ Homo sapiens ]
Official Symbol: DPPA2
Synonyms: DPPA2; developmental pluripotency associated 2; developmental pluripotency-associated protein 2; cancer/testis antigen 100; CT100; PESCRG1; embryonic stem cell (ESC) associated transcript 15-2; pluripotent embryonic stem cell-related gene 1 protein; ECAT15-2;
Gene ID: 151871
mRNA Refseq: NM_138815
Protein Refseq: NP_620170
MIM: 614445
UniProt ID: Q7Z7J5
Chromosome Location: 3q13.13
Function: nucleic acid binding;

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