"DNM3" Related Products

Recombinant Human DNM3 293 Cell Lysate

Cat.No.: DNM3-6856HCL
Description: Antigen standard for dynamin 3 (DNM3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DNM3 dynamin 3 [ Homo sapiens ]
Official Symbol: DNM3
Synonyms: DNM3; dynamin 3; dynamin-3; Dyna III; KIAA0820; T-dynamin; dynamin, testicular; dynamin family member; MGC70433;
Gene ID: 26052
mRNA Refseq: NM_001136127
Protein Refseq: NP_001129599
MIM: 611445
UniProt ID: Q9UQ16
Chromosome Location: 1q24.1
Pathway: Axon guidance, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Developmental Biology, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, conserved biosystem; Endocytosis, organism-specific biosystem;
Function: GTP binding; GTPase activity; GTPase activity; hydrolase activity; nucleotide binding; protein binding;

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