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Recombinant Human DLD 293 Cell Lysate

Cat.No.: DLD-6912HCL
Description: Antigen standard for dihydrolipoamide dehydrogenase (DLD) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DLD dihydrolipoamide dehydrogenase [ Homo sapiens ]
Official Symbol: DLD
Synonyms: DLD; dihydrolipoamide dehydrogenase; dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2 oxo glutarate complex, branched chain keto acid dehydrogenase complex) , GCSL, LAD; dihydrolipoyl dehydrogenase, mitochondrial; DLDH; E3 component of pyruvate dehydrogenase complex; 2 oxo glutarate complex; branched chain keto acid dehydrogenase complex; diaphorase; lipoamide reductase; lipoyl dehydrogenase; lipoamide dehydrogenase; glycine cleavage system L protein; glycine cleavage system protein L; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex; E3; LAD; GCSL; PHE3;
Gene ID: 1738
mRNA Refseq: NM_000108
Protein Refseq: NP_000099
MIM: 238331
UniProt ID: P09622
Chromosome Location: 7q31-q32
Pathway: 2-ketoglutarate dehydrogenase complex, organism-specific biosystem; 2-ketoglutarate dehydrogenase complex, conserved biosystem; 2-oxobutanoate degradation I, organism-specific biosystem; 2-oxobutanoate degradation I, conserved biosystem; Branched-chain amino acid catabolism, organism-specific biosystem; Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem;
Function: NAD binding; dihydrolipoyl dehydrogenase activity; flavin adenine dinucleotide binding; lipoamide binding; oxidoreductase activity;

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