Human DLD Knockdown Cell Lysate

• Cat.No.: DLD-138HKCL
• Product Overview: WB-validated DLD Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 54 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg DLD KD HeLa cell lysate
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glycine, Glycolysis / Gluconeogenesis, leucine and isoleucine degradation, Metabolic pathways, Pyruvate metabolism, serine and threonine metabolism, Valine
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: DLD dihydrolipoamide dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: DLD
• Synonyms: DLD; dihydrolipoamide dehydrogenase; dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2 oxo glutarate complex, branched chain keto acid dehydrogenase complex), GCSL, LAD; dihydrolipoyl dehydrogenase, mitochondrial; DLDH; E3 component of pyruvate dehydrogenase complex; 2 oxo glutarate complex; branched chain keto acid dehydrogenase complex; diaphorase; lipoamide reductase; lipoyl dehydrogenase; lipoamide dehydrogenase; glycine cleavage system L protein; glycine cleavage system protein L; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex; E3; LAD; GCSL; PHE3
• Gene ID: 1738
• mRNA Refseq: NM_000108
• Protein Refseq: NP_000099
• MIM: 238331
• UniProt ID: P09622