"DEPDC6" Related Products

Recombinant Human DEPDC6 293 Cell Lysate

Cat.No.: DEPDC6-6973HCL
Description: Antigen standard for DEP domain containing 6 (DEPDC6) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DEPTOR?DEP domain containing MTOR-interacting protein [?Homo sapiens?(human) ]
Official Symbol: DEPDC6
Synonyms: DEPTOR; DEP domain containing MTOR-interacting protein; DEP.6; DEPDC6; DEP domain-containing mTOR-interacting protein; DEP domain containing 6; DEP domain-containing protein 6; DEP domain containing mTOR interacting protein
Gene ID: 64798
mRNA Refseq: NM_022783
Protein Refseq: NP_073620
MIM: 612974
UniProt ID: Q8TB45
Chromosome Location: 8q24.12
Pathway: Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met), organism-specific biosystem; mTOR signaling pathway, organism-specific biosystem
Function: protein binding
Products Types ◆ Recombinant Protein

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