"DDX28" Related Products

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Recombinant Human DDX28 293 Cell Lysate

Cat.No.: DDX28-7011HCL
Description: Antigen standard for DEAD (Asp-Glu-Ala-Asp) box polypeptide 28 (DDX28), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DDX28 DEAD (Asp-Glu-Ala-Asp) box polypeptide 28 [ Homo sapiens ]
Official Symbol: DDX28
Synonyms: DDX28; DEAD (Asp-Glu-Ala-Asp) box polypeptide 28; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 28; probable ATP-dependent RNA helicase DDX28; FLJ11282; MDDX28; mitochondrial DEAD box protein 28; mitochondrial DEAD-box polypeptide 28; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28;
Gene ID: 55794
mRNA Refseq: NM_018380
Protein Refseq: NP_060850
MIM: 607618
UniProt ID: Q9NUL7
Chromosome Location: 16q22.1-q22.3
Function: ATP binding; ATP-dependent helicase activity; RNA binding; helicase activity; hydrolase activity; nucleotide binding;

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