"DDX1" Related Products

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Recombinant Human DDX1 293 Cell Lysate

Cat.No.: DDX1-7021HCL
Description: Antigen standard for DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DDX1 DEAD (Asp-Glu-Ala-Asp) box helicase 1 [ Homo sapiens ]
Official Symbol: DDX1
Synonyms: DDX1; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp Glu Ala Asp) box polypeptide 1 , DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1; ATP-dependent RNA helicase DDX1; DBP RB; DEAD box-1; DEAD box protein 1; DEAD box polypeptide 1; DEAD box protein retinoblastoma; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1; DBP-RB; UKVH5d;
Gene ID: 1653
mRNA Refseq: NM_004939
Protein Refseq: NP_004930
MIM: 601257
UniProt ID: Q92499
Chromosome Location: 2p24
Pathway: mRNA processing, organism-specific biosystem;
Function: ATP binding; ATP-dependent helicase activity; DNA binding; DNA/RNA helicase activity; RNA binding; RNA helicase activity; chromatin binding; exonuclease activity; hydrolase activity; nuclease activity; nucleotide binding; poly(A) RNA binding; protein binding; transcription cofactor activity;

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