"DCP1A" Related Products

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Recombinant Human DCP1A 293 Cell Lysate

Cat.No.: DCP1A-7048HCL
Description: Antigen standard for DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DCP1A DCP1 decapping enzyme homolog A (S. cerevisiae) [ Homo sapiens ]
Official Symbol: DCP1A
Synonyms: DCP1A; DCP1 decapping enzyme homolog A (S. cerevisiae); mRNA-decapping enzyme 1A; HSA275986; SMAD4IP1; SMIF; decapping enzyme hDcp1a; transcription factor SMIF; putative protein product of Nbla00360; Smad4-interacting transcriptional co-activator; Nbla00360; FLJ21691;
Gene ID: 55802
mRNA Refseq: NM_018403
Protein Refseq: NP_060873
MIM: 607010
UniProt ID: Q9NPI6
Chromosome Location: 3p21.1
Pathway: Deadenylation-dependent mRNA decay, organism-specific biosystem; Decapping complex, organism-specific biosystem; Decapping complex, conserved biosystem; Destabilization of mRNA by Butyrate Response Factor 1 (BRF1), organism-specific biosystem; Destabilization of mRNA by Tristetraprolin (TTP), organism-specific biosystem; Gene Expression, organism-specific biosystem; RNA degradation, organism-specific biosystem;
Function: hydrolase activity; identical protein binding; protein binding;

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