"DCAF4" Related Products

Recombinant Human DCAF4 293 Cell Lysate

Cat.No.: DCAF4-7056HCL
Description: Antigen standard for DDB1 and CUL4 associated factor 4 (DCAF4), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DCAF4 DDB1 and CUL4 associated factor 4 [ Homo sapiens ]
Official Symbol: DCAF4
Synonyms: DCAF4; DDB1 and CUL4 associated factor 4; WD repeat domain 21 , WD repeat domain 21A , WDR21, WDR21A; DDB1- and CUL4-associated factor 4; DKFZp434K114; WD repeat domain 21A; WD repeat-containing protein 21A; WDR21; WDR21A; MGC20547; MGC46524;
Gene ID: 26094
mRNA Refseq: NM_001163508
Protein Refseq: NP_001156980
UniProt ID: Q8WV16
Chromosome Location: 14q24.3

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