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Recombinant Human DAPP1 293 Cell Lysate

Cat.No. : DAPP1-7074HCL
  • Specification
  • Gene Information
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Description : Antigen standard for dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : DAPP1 dual adaptor of phosphotyrosine and 3-phosphoinositides [ Homo sapiens ]
Official Symbol : DAPP1
Synonyms : DAPP1; dual adaptor of phosphotyrosine and 3-phosphoinositides; dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; BAM32; hDAPP1; B-cell adapter molecule of 32 kDa; b lymphocyte adapter protein Bam32; DKFZp667E0716;
Gene ID : 27071
mRNA Refseq : NM_014395
Protein Refseq : NP_055210
MIM : 605768
UniProt ID : Q9UN19
Chromosome Location : 4q25-q27
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; BCR signaling pathway, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem;
Function : phosphatidylinositol-3,4,5-trisphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phospholipid binding; protein tyrosine phosphatase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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