Recombinant Human CYP2E1 293 Cell Lysate CYP2E1-7111HCL

Recombinant Human CYP2E1 293 Cell Lysate

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Recombinant Human CYP2E1 293 Cell Lysate

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Cat.No. : CYP2E1-7111HCL
Description : Antigen standard for cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 [ Homo sapiens (human) ]
Official Symbol : CYP2E1
Synonyms : CYP2E1; CPE1; CYP2E; P450-J; P450C2E; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450 2E1; CYPIIE1; cytochrome P450-J; microsomal monooxygenase; xenobiotic monooxygenase; 4-nitrophenol 2-hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; NP_000764.1; EC 1.14.13.n7
Gene ID : 1571
mRNA Refseq : NM_000773
Protein Refseq : NP_000764
MIM : 124040
UniProt ID : P05181
Chromosome Location : 10q26.3
Pathway : Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; CYP2E1 reactions, organism-specific biosystem; Chemical carcinogenesis, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem
Function : enzyme binding; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity;; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding

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