Recombinant Human CYP27B1 293 Cell Lysate

• Cat.No.: CYP27B1-7118HCL

Specification

• Description: Antigen standard for cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CYP27B1 cytochrome P450, family 27, subfamily B, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP27B1
• Synonyms: CYP27B1; cytochrome P450, family 27, subfamily B, polypeptide 1; cytochrome P450, subfamily XXVIIB (25 hydroxyvitamin D 1 alpha hydroxylase), polypeptide 1 , PDDR, VDD1; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 1alpha(OH)ase; 25 Hydroxyvitamin D3 1alpha hydroxylase; CYP1; P450c1; VDDR I; VD3 1A hydroxylase; cytochrome p450 27B1; cytochrome P450C1 alpha; cytochrome P450VD1-alpha; calcidiol 1-monooxygenase; 25-OHD-1 alpha-hydroxylase; 25 hydroxyvitamin D3-1-alpha hydroxylase; cytochrome P450 subfamily XXVIIB polypeptide 1; VDR; CP2B; PDDR; VDD1; VDDR; VDDRI; CYP27B; CYP1alpha
• Gene ID: 1594
• mRNA Refseq: NM_000785
• Protein Refseq: NP_000776
• MIM: 609506
• UniProt ID: O15528
• Chromosome Location: 12q13.3-q14
• Pathway: 1,25-dihydroxyvitamin D3 biosynthesis, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Cholecalciferol biosynthesis, organism-specific biosystem; Cholecalciferol biosynthesis, conserved biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: calcidiol 1-monooxygenase activity; electron carrier activity; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen