Recombinant Human CYP26A1 293 Cell Lysate

• Cat.No.: CYP26A1-7121HCL

Specification

• Description: Antigen standard for cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CYP26A1 cytochrome P450, family 26, subfamily A, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP26A1
• Synonyms: CYP26A1; cytochrome P450, family 26, subfamily A, polypeptide 1; cytochrome P450, subfamily XXVIA, polypeptide 1; cytochrome P450 26A1; CP26; CYP26; P450RAI; P450RAI1; hP450RAI; cytochrome P450RAI; retinoic acid 4-hydroxylase; P450, retinoic acid-inactivating, 1; retinoic acid-metabolizing cytochrome; cytochrome P450 retinoic acid-inactivating 1
• Gene ID: 1592
• mRNA Refseq: NM_000783
• Protein Refseq: NP_000774
• MIM: 602239
• UniProt ID: O43174
• Chromosome Location: 10q23-q24
• Pathway: Adipogenesis, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Metabolism, organism-specific biosystem; Nuclear receptors in lipid metabolism and toxicity, organism-specific biosystem; Phase 1 - Functionalization of compounds, organism-specific biosystem; Retinol metabolism, organism-specific biosystem
• Function: electron carrier activity; heme binding; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; oxygen binding; retinoic acid 4-hydroxylase activity; retinoic acid binding