Recombinant Human CSTF1 293 Cell Lysate

• Cat.No.: CSTF1-7223HCL

Specification

• Description: Antigen standard for cleavage stimulation factor, 3 pre-RNA, subunit 1, 50kDa (CSTF1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CSTF1 cleavage stimulation factor, 3 pre-RNA, subunit 1, 50kDa [ Homo sapiens ]
• Official Symbol: CSTF1
• Synonyms: CSTF1; cleavage stimulation factor, 3 pre-RNA, subunit 1, 50kDa; cleavage stimulation factor, 3 pre RNA, subunit 1, 50kD; cleavage stimulation factor subunit 1; CF-1 50 kDa subunit; CSTF 50 kDa subunit; cleavage stimulation factor 50 kDa subunit; CstF-50; CstFp50
• Gene ID: 1477
• mRNA Refseq: NM_001033521
• Protein Refseq: NP_001028693
• MIM: 600369
• UniProt ID: Q05048
• Chromosome Location: 20q13.31
• Pathway: BARD1 signaling events, organism-specific biosystem; Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Processing of Capped Intronless Pre-mRNA, organism-specific biosystem; Processing of Intronless Pre-mRNAs, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem
• Function: RNA binding; protein binding