Recombinant Human CRLF2 293 Cell Lysate

• Cat.No.: CRLF2-7275HCL

Specification

• Description: Antigen standard for cytokine receptor-like factor 2 (CRLF2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CRLF2 cytokine receptor-like factor 2 [ Homo sapiens ]
• Official Symbol: CRLF2
• Synonyms: CRLF2; cytokine receptor-like factor 2; CRL2; TSLPR; IL-XR; TSLP receptor; P2RY8/CRLF2 fusion; cytokine receptor-like 2; cytokine receptor CRL2 precusor; thymic stromal lymphopoietin receptor; thymic stromal lymphopoietin protein receptor; thymic stromal-derived lymphopoietin receptor; CRLF2Y
• Gene ID: 64109
• mRNA Refseq: NM_001012288
• Protein Refseq: NP_001012288
• MIM: 300357
• UniProt ID: Q9HC73
• Chromosome Location: Xp22.3 and Yp11.3
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: receptor activity

Reviews

03/02/2023

CRLF2 is highly expressed, which is convenient for detection and quantification.

06/11/2022

Green and pollution-free, it can be seen that the manufacturer is very environmentally conscious.

10/01/2020

The range of applications is breathtaking, and it can play an important role in any field.

Q&As

How does CRLF2 protein interact with other proteins?

The protein may interact with other proteins to fulfill its function. These interactions can involve various types of protein-protein interactions like electrostatic interactions, hydrogen bonding, etc. Understanding these interactions can help unravel the mechanism of action of the CRLF2 protein in cells.

How is the expression level of CRLF2 protein regulated?

The expression level of CRLF2 protein may be regulated by a variety of factors, including gene transcription, translation, post-transcriptional modification, etc. Studying these regulatory mechanisms can help to understand the expression patterns of CRLF2 protein in organisms and its impact on biological processes.

What are the effects of mutations or modifications in the CRLF2 protein on organisms?

Mutations or modifications of the CRLF2 protein may affect its function and thus affect the organism. The specific effects may vary depending on the type and location of the mutation or modification and may involve various biological processes, such as growth and development, immune response, etc.

In which biological processes does CRLF2 protein play a role?

CRLF2 protein may be involved in a variety of biological processes, including immune response, regulation of hematopoietic processes, cell growth and differentiation, etc. These processes require the regulatory effect of CRLF2 protein for normal life activities.

What is the structure and function of CRLF2 protein?

Studying the structure and function of CRLF2 protein may require the design of a series of experiments, including biochemical and cell biology experiments. For example, X-ray crystal diffraction can be used to study its structure, and its function can be studied by gene knockout or knockdown techniques.

How to evaluate the potential of CRLF2 protein as a drug target?

Evaluating the potential of CRLF2 protein as a drug target may involve several aspects, including its expression level in organisms, its role in biological processes, and its changes in disease. In addition, factors such as ease of drug development for this protein and potential drug side effects need to be considered. At the same time, in vitro and in vivo experiments can be performed with known CRLF2 inhibitors or agonists to verify their efficacy and safety.