"CPA3" Related Products

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Recombinant Human CPA3 293 Cell Lysate

Cat.No.: CPA3-7321HCL
Description: Antigen standard for carboxypeptidase A3 (mast cell) (CPA3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CPA3 carboxypeptidase A3 (mast cell) [ Homo sapiens ]
Official Symbol: CPA3
Synonyms: CPA3; carboxypeptidase A3 (mast cell); mast cell carboxypeptidase A; tissue carboxypeptidase A; MC-CPA;
Gene ID: 1359
mRNA Refseq: NM_001870
Protein Refseq: NP_001861
MIM: 114851
UniProt ID: P15088
Chromosome Location: 3q24
Pathway: Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem; Renin-angiotensin system, organism-specific biosystem; Renin-angiotensin system, conserved biosystem;
Function: metal ion binding; metallocarboxypeptidase activity; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding;

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