Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human COMT 293 Cell Lysate

Cat.No. : COMT-7365HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for catechol-O-methyltransferase (COMT), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : COMT catechol-O-methyltransferase [ Homo sapiens ]
Official Symbol : COMT
Synonyms : COMT; catechol-O-methyltransferase; catechol O-methyltransferase;
Gene ID : 1312
mRNA Refseq : NM_000754
Protein Refseq : NP_000745
UniProt ID : P21964
Chromosome Location : 22q11.21
Pathway : Biogenic Amine Synthesis, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Dopamine clearance from the synaptic cleft, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem; Enzymatic degradation of Dopamine by monoamine oxidase, organism-specific biosystem; Enzymatic degradation of dopamine by COMT, organism-specific biosystem;
Function : O-methyltransferase activity; catechol O-methyltransferase activity; magnesium ion binding; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (3)

Write a review
Reviews
07/06/2021

    Its purity, stability, and functionality make it an ideal choice for my research endeavors.

    09/16/2019

      The COMT protein offered by the manufacturer exhibits exceptional quality and is precisely tailored to meet my experimental requirements.

      04/09/2016

        Manufacturers should ensure the consistency and quality of COMT protein products.

        Ask a Question for All COMT Products

        Required fields are marked with *

        My Review for All COMT Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends