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Recombinant Human CISH 293 Cell Lysate

Cat.No.: CISH-7488HCL
Description: Antigen standard for cytokine inducible SH2-containing protein (CISH), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CISH cytokine inducible SH2-containing protein [ Homo sapiens ]
Official Symbol: CISH
Synonyms: CISH; cytokine inducible SH2-containing protein; cytokine-inducible SH2-containing protein; CIS; CIS 1; G18; SOCS; suppressor of cytokine signaling; cytokine-inducible inhibitor of signaling type 1B; CIS-1; BACTS2;
Gene ID: 1154
mRNA Refseq: NM_013324
Protein Refseq: NP_037456
MIM: 602441
UniProt ID: Q9NSE2
Chromosome Location: 3p21.3
Pathway: Cytokine Signaling in Immune system, organism-specific biosystem; ECS complex, organism-specific biosystem; ECS complex, conserved biosystem; EPO Receptor Signaling, organism-specific biosystem; Growth hormone receptor signaling, organism-specific biosystem; IL-2 Signaling Pathway, organism-specific biosystem; IL-3 Signaling Pathway, organism-specific biosystem;
Function: molecular_function;

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