Recombinant Human CIB1 293 Cell Lysate


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Recombinant Human CIB1 293 Cell Lysate

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Cat.No. : CIB1-7499HCL
Description : Antigen standard for calcium and integrin binding 1 (calmyrin) (CIB1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CIB1 calcium and integrin binding 1 (calmyrin) [ Homo sapiens ]
Official Symbol : CIB1
Synonyms : CIB1; calcium and integrin binding 1 (calmyrin); calcium and integrin-binding protein 1; CALMYRIN; CIB; KIP; SIP2 28; CIBP; calmyrin; kinase-interacting protein; DNA-PKcs-interacting protein; SNK-interacting protein 2-28; Snk interacting protein 2-28; calcium- and integrin-binding protein; calcium and integrin binding protein 1; DNA-dependent protein kinase interacting protein; calcium and integrin binding, protein kinase interacting protein; KIP1; SIP2-28;
Gene ID : 10519
mRNA Refseq : NM_006384
Protein Refseq : NP_006375
MIM : 602293
UniProt ID : Q99828
Chromosome Location : 15q25.3-q26
Function : calcium ion binding; protein binding;

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