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Recombinant Human CHIA 293 Cell Lysate

Cat.No. : CHIA-7538HCL
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  • Gene Information
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Description : Antigen standard for chitinase, acidic (CHIA), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CHIA chitinase, acidic [ Homo sapiens ]
Official Symbol : CHIA
Synonyms : CHIA; chitinase, acidic; acidic mammalian chitinase; AMCase; CHIT2; TSA1902; lung-specific protein TSA1902; AMCASE; DKFZp313J1722;
Gene ID : 27159
mRNA Refseq : NM_201653
Protein Refseq : NP_970615
MIM : 606080
UniProt ID : Q9BZP6
Chromosome Location : 1p13.2
Pathway : Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem;
Function : catalytic activity; cation binding; chitin binding; chitin binding; chitinase activity; chitinase activity; chitinase activity; kinase binding; lysozyme activity; sugar binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What are the mechanisms by which CHIA modulates gut microbiota and gastrointestinal health? 11/14/2022

CHIA may affect the gut microbiota and gastrointestinal health, influencing digestive processes and gut immunity.

How does CHIA affect lung function and respiratory diseases beyond asthma? 09/23/2021

CHIA impacts lung function, with potential roles in various respiratory diseases beyond asthma.

How does CHIA influence inflammatory processes, particularly in the context of chronic inflammation? 04/26/2020

CHIA influences chronic inflammatory responses, possibly exacerbating or modulating the inflammation.

What role does CHIA play in allergic reactions and asthma pathogenesis? 07/10/2019

CHIA is implicated in allergic reactions and asthma, potentially contributing to inflammation in the airways.

What is the impact of CHIA on the immune response to parasitic infections? 06/17/2019

CHIA aids in the immune response against parasitic infections by breaking down chitin found in parasite structures.

How do alterations in CHIA expression or activity affect tissue remodeling and fibrosis? 05/19/2018

Variations in CHIA expression or activity can influence tissue remodeling and the development of fibrosis.

How does CHIA (Chitinase 3 Like 1) contribute to the breakdown of chitin and other polysaccharides in the body? 02/07/2018

CHIA helps break down chitin and similar polysaccharides, playing a role in digestion and pathogen defense.

Customer Reviews (3)

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Reviews
03/31/2022

    Efficient protein purification, cost-effective, and dependable.

    09/09/2019

      Precise protein quantification, ensures data accuracy in our research.

      10/07/2018

        Dependable peptide synthesis, accelerates our peptide-based projects effectively.

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