"CES5A" Related Products

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Recombinant Human CES7 293 Cell Lysate

Cat.No.: CES5A-7563HCL
Description: Antigen standard for carboxylesterase 7 (CES7), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CES5A?carboxylesterase 5A [?Homo sapiens?(human) ]
Official Symbol: CES5A
Synonyms: CES5A; carboxylesterase 5A; CES5; CES7; CAUXIN; CES4C1; HEL126; carboxylesterase 5A; carboxylesterase 5; carboxylesterase 7; epididymis luminal protein 126; carboxylesterase-like urinary excreted protein homolog; NP_001137157.1; EC 3.1.1.1; NP_001177087.1; NP_001177087.1
Gene ID: 221223
mRNA Refseq: NM_145024
Protein Refseq: NP_659461
UniProt ID: Q6NT32
Chromosome Location: 16q12.2
Pathway: E2F transcription factor network, organism-specific biosystem; Phase I, non P450, organism-specific biosystem; retinol biosynthesis, organism-specific biosystem
Function: carboxylic ester hydrolase activity

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