Recombinant Human CEP57 293 Cell Lysate

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Recombinant Human CEP57 293 Cell Lysate

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Cat.No. : CEP57-7572HCL
Description : Antigen standard for centrosomal protein 57kDa (CEP57) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CEP57 centrosomal protein 57kDa [ Homo sapiens ]
Official Symbol : CEP57
Synonyms : CEP57; centrosomal protein 57kDa; centrosomal protein of 57 kDa; KIAA0092; Translokin; TSP57; translokin; FGF2-interacting protein; testis-specific protein 57; proliferation-inducing protein 8; MVA2; PIG8;
Gene ID : 9702
mRNA Refseq : NM_001243776
Protein Refseq : NP_001230705
MIM : 607951
UniProt ID : Q86XR8
Chromosome Location : 11q21
Pathway : Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; G2/M Transition, organism-specific biosystem; Loss of Nlp from mitotic centrosomes, organism-specific biosystem; Loss of proteins required for interphase microtubule organization??from the centrosome, organism-specific biosystem; Mitotic G2-G2/M phases, organism-specific biosystem;
Function : fibroblast growth factor binding; microtubule binding; protein binding; protein homodimerization activity;

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