"CENPV" Related Products

Recombinant Human CENPV 293 Cell Lysate

Cat.No.: CENPV-7574HCL
Description: Antigen standard for centromere protein V (CENPV) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CENPV centromere protein V [ Homo sapiens ]
Official Symbol: CENPV
Synonyms: CENPV; centromere protein V; proline rich 6 , PRR6; CENP V; p30; proline rich 6; nuclear protein p30; proline-rich protein 6; PRR6; CENP-V; 3110013H01Rik;
Gene ID: 201161
mRNA Refseq: NM_181716
Protein Refseq: NP_859067
MIM: 608139
UniProt ID: Q7Z7K6
Chromosome Location: 17p11.2
Function: carbon-sulfur lyase activity; molecular_function;

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