Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human CDK7 293 Cell Lysate

Cat.No. : CDK7-7621HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for cyclin-dependent kinase 7 (CDK7) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CDK7 cyclin-dependent kinase 7 [ Homo sapiens ]
Official Symbol : CDK7
Synonyms : CDK7; cyclin-dependent kinase 7; cyclin dependent kinase 7 (homolog of Xenopus MO15 cdk activating kinase) , cyclin dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk activating kinase); CAK; CAK1; CDKN7; MO15; STK1; p39 Mo15; protein kinase; 39 KDa protein kinase; kinase subunit of CAK; CDK-activating kinase 1; serine/threonine kinase stk1; cell division protein kinase 7; serine/threonine protein kinase 1; serine/threonine-protein kinase 1; serine/threonine protein kinase MO15; homolog of Xenopus MO15 Cdk-activating kinase; TFIIH basal transcription factor complex kinase subunit; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); HCAK; p39MO15;
Gene ID : 1022
mRNA Refseq : NM_001799
Protein Refseq : NP_001790
MIM : 601955
UniProt ID : P50613
Chromosome Location : 5q12.1
Pathway : Androgen Receptor Signaling Pathway, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem;
Function : ATP binding; DNA-dependent ATPase activity; RNA polymerase II carboxy-terminal domain kinase activity; androgen receptor binding; cyclin-dependent protein kinase activity; nucleotide binding; protein C-terminus binding; protein complex binding; protein kinase activity; transcription coactivator activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (6)

Ask a question
What drugs can act on CDK7? 11/21/2022

At present, some drugs have been developed to act on CDK7, including some small molecule inhibitors and antibody drugs. These drugs can inhibit the activity of CDK7 or regulate its expression, thereby treating diseases associated with aberrant expression of CDK7.

What are the treatment strategies for CDK7? 10/16/2021

Therapeutic strategies for CDK7 include inhibition of its activity, regulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against CDK7 to inhibit its aberrant activity in tumors, or the regulation of CDK7 expression through gene knockout or overexpression technologies.

What factors can affect the expression of CDK7? 10/11/2021

The expression of CDK7 is affected by a variety of factors, including transcription factors, epigenetic modifications, and hormones. For example, the E2F transcription factor can promote the transcription of CDK7, while the histone methyltransferase EZH2 can regulate the cell cycle by inhibiting the transcription of CDK7.

Which biomolecules can be used as molecular markers for CDK7? 05/17/2021

Certain biomolecules can be used as molecular markers of CDK7, such as the mRNA or protein of CDK7 detected in biological samples such as blood and urine. These markers can be used for early diagnosis of disease, prognostic judgment, or monitoring of treatment efficacy.

What is the role of CDK7 in the cell cycle? 11/20/2020

The main role of CDK7 in the cell cycle is to activate CDK2 and CDK1, which in turn drive the cell from G1 to S phase and from G2 to M phase. It regulates cell cycle progression by phosphorylating a variety of substrates, including Rb protein and Wee1 kinase.

How is the activity of CDK7 detected? 08/29/2020

CDK7 activity can be detected by methods such as phosphowestern blotting, kinase activity assays, and cell cycle analysis. These methods can detect the phosphorylation level and cell cycle distribution of CDK7 to assess its activity status in cells.

Customer Reviews (3)

Write a review
Reviews
08/14/2022

    We are very satisfied with the performance and quality of their products and will continue to buy them.

    07/07/2022

      It has high catalysis, high safety and high expression level, which makes the experimental process more convenient and saves a lot of time for scientific research.

      11/25/2021

        The fluorescence and absorption properties are very good.

        Ask a Question for All CDK7 Products

        Required fields are marked with *

        My Review for All CDK7 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends