"CDC5L" Related Products

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Recombinant Human CDC5L 293 Cell Lysate

Cat.No.: CDC5L-7648HCL
Description: Antigen standard for CDC5 cell division cycle 5-like (S. pombe) (CDC5L) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CDC5L CDC5 cell division cycle 5-like (S. pombe) [ Homo sapiens ]
Official Symbol: CDC5L
Synonyms: CDC5L; CDC5 cell division cycle 5-like (S. pombe); CDC5 (cell division cycle 5, S. pombe, homolog) like; cell division cycle 5-like protein; CDC5; CEF1; hCDC5; PCDC5RP; Cdc5-related protein; pombe cdc5-related protein; dJ319D22.1 (CDC5-like protein); CDC5-LIKE; dJ319D22.1; KIAA0432;
Gene ID: 988
mRNA Refseq: NM_001253
Protein Refseq: NP_001244
MIM: 602868
UniProt ID: Q99459
Chromosome Location: 6p
Pathway: Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; Spliceosome, 35S U5-snRNP, organism-specific biosystem; Spliceosome, Prp19/CDC5L complex, organism-specific biosystem;
Function: DNA binding; RNA binding; protein binding; protein domain specific binding;

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