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Recombinant Human CDC40 293 Cell Lysate

Cat.No.: CDC40-7658HCL
Description: Antigen standard for cell division cycle 40 homolog (S. cerevisiae) (CDC40) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CDC40 cell division cycle 40 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: CDC40
Synonyms: EHB3; PRP17; PRPF17
Gene ID: 51362
mRNA Refseq: NM_015891.2
Protein Refseq: NP_056975.1
MIM: 605585
UniProt ID: O60508
Chromosome Location: 6q21
Pathway: Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem;Gene Expression, organism-specific biosystem;Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem;RNA Polymerase II Transcription, organism-specific biosystem;RNA Polymerase II Transcription Termination, organism-specific biosystem;Spliceosome, organism-specific biosystem;Spliceosome, conserved biosystem;

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