Recombinant Human CDC25B 293 Cell Lysate

• Cat.No.: CDC25B-7665HCL

Specification

• Description: Antigen standard for cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CDC25B cell division cycle 25 homolog B (S. pombe) [ Homo sapiens ]
• Official Symbol: CDC25B
• Synonyms: CDC25B; cell division cycle 25 homolog B (S. pombe); cell division cycle 25 homolog B (S. cerevisiae) , cell division cycle 25B; M-phase inducer phosphatase 2; cell division cycle 25B; dual specificity phosphatase Cdc25B
• Gene ID: 994
• mRNA Refseq: NM_021872
• Protein Refseq: NP_068658
• MIM: 116949
• UniProt ID: P30305
• Chromosome Location: 20p13
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem
• Function: hydrolase activity; protein kinase binding; protein tyrosine phosphatase activity

Reviews

07/05/2022

Consistency defines the reagent's performance, offering remarkable advantages for my scientific investigations.

12/10/2020

Precise outcomes have been yielded by employing this protein reagent, aligning flawlessly with the requirements of my experiments.

08/01/2020

The manufacturer's professionalism truly shines, instilling unwavering trustworthiness.