Recombinant Human CCT3 293 Cell Lysate

• Cat.No.: CCT3-7690HCL

Specification

• Description: Antigen standard for chaperonin containing TCP1, subunit 3 (gamma) (CCT3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CCT3 chaperonin containing TCP1, subunit 3 (gamma) [ Homo sapiens ]
• Official Symbol: CCT3
• Synonyms: CCT3; chaperonin containing TCP1, subunit 3 (gamma); TRIC5; T-complex protein 1 subunit gamma; Cctg; hTRiC5; T-complex protein 1, gamma subunit; TCP1 (t-complex-1) ring complex, polypeptide 5; CCTG; PIG48; CCT-gamma; TCP-1-gamma
• Gene ID: 7203
• mRNA Refseq: NM_005998
• Protein Refseq: NP_005989
• MIM: 600114
• UniProt ID: P49368
• Chromosome Location: 1q23
• Pathway: Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystem; Chaperonin-mediated protein folding, organism-specific biosystem; Cooperation of Prefoldin and TriC/CCTin actin and tubulin folding, organism-specific biosystem; Folding of actin by CCT/TriC, organism-specific biosystem; Formation of tubulin folding intermediates by CCT/TriC, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; Prefoldin mediated transfer of substrateto CCT/TriC, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; unfolded protein binding