"CAPN3" Related Products


Recombinant Human CAPN3 293 Cell Lysate

Cat.No.: CAPN3-7862HCL
Description: Antigen standard for calpain 3, (p94) (CAPN3), transcript variant 6 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CAPN3 calpain 3, (p94) [ Homo sapiens ]
Official Symbol: CAPN3
Synonyms: CAPN3; calpain 3, (p94); LGMD2, LGMD2A; calpain-3; CANP3; nCL 1; p94; calpain L3; new calpain 1; calpain, large polypeptide L3; calcium-activated neutral proteinase 3; calpain p94, large [catalytic] subunit; muscle-specific calcium-activated neutral protease 3 large subunit; LGMD2; nCL-1; CANPL3; LGMD2A; MGC4403; MGC10767; MGC11121; MGC14344;
Gene ID: 825
mRNA Refseq: NM_000070
Protein Refseq: NP_000061
MIM: 114240
UniProt ID: P20807
Chromosome Location: 15q15.1
Pathway: Integrin-mediated cell adhesion, organism-specific biosystem; TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem;
Function: calcium ion binding; calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; calcium-dependent cysteine-type endopeptidase activity; calcium-dependent cysteine-type endopeptidase activity; catalytic activity; cysteine-type peptidase activity; ligase regulator activity; peptidase activity; peptidase activity; protein complex scaffold; signal transducer activity; sodium ion binding; structural constituent of muscle; titin binding;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.