Recombinant Human CALB2 293 Cell Lysate

• Cat.No.: CALB2-7896HCL

Specification

• Description: Antigen standard for calbindin 2 (CALB2), transcript variant CALB2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CALB2 calbindin 2 [ Homo sapiens ]
• Official Symbol: CALB2
• Synonyms: CALB2; calbindin 2; calbindin 2, 29kDa (calretinin); calretinin; CAL2; calbindin D29K; 29 kDa calbindin; calbindin 2, (29kD, calretinin); CR; CAB29
• Gene ID: 794
• mRNA Refseq: NM_001740
• Protein Refseq: NP_001731
• MIM: 114051
• UniProt ID: P22676
• Chromosome Location: 16q22.2
• Function: calcium ion binding

Reviews

10/19/2021

High-quality recombinant proteins boost research productivity.

06/13/2019

Ease of use makes them ideal for both novice and expert researchers.

07/07/2018

They are essential components of our lab toolkit.

Q&As

Are there any therapeutic strategies targeting CALB2 for neurological conditions?

While there are no specific drugs targeting CALB2, research into calcium homeostasis modulation and CALB2's role in disease may lead to potential therapeutic avenues.

What is the role of CALB2 in the maintenance of neuronal calcium gradients across subcellular compartments, and how does this impact cellular function?

CALB2 helps establish and maintain calcium gradients within neurons, ensuring proper functioning of processes such as neurotransmission, dendritic integration, and synaptic plasticity by fine-tuning local calcium concentrations.

How does CALB2 contribute to calcium-dependent gene regulation within neurons at the molecular level?

CALB2 can interact with transcription factors and co-factors involved in calcium-dependent gene expression, influencing the activation or repression of specific genes in response to changes in intracellular calcium levels.

How does CALB2 function in balancing calcium levels during neuronal activity and synaptic transmission at the molecular level?

CALB2 dynamically binds and releases calcium ions in response to changes in intracellular calcium concentration, fine-tuning calcium signaling during neuronal activity and synaptic transmission.

In which specific neuronal cell types and brain regions is CALB2 highly expressed?

CALB2 is abundantly expressed in various neuronal populations, including cerebellar granule cells, interneurons in the cortex, and certain subtypes of neurons in the hippocampus.