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Recombinant Human CACNB4 293 Cell Lysate

Cat.No.: CACNB4-7902HCL
Description: Antigen standard for calcium channel, voltage-dependent, beta 4 subunit (CACNB4), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CACNB4 calcium channel,voltage-dependent,beta 4 subunit [ Homo sapiens ]
Official Symbol: CACNB4
Synonyms: CACNB4;Cavβ4 Calcium Channel;EA5;EJM;calcium channel,voltage-dependent,beta 4 subunit;CAB4;EIG9;EJM4;EJM6;CACNLB4;voltage-dependent L-type calcium channel subunit beta-4;calcium channel voltage-dependent subunit beta 4;dihydropyridine-sensitive L-type,cal
Gene ID: 785
mRNA Refseq: NM_000726
Protein Refseq: NP_000717
MIM: 601949
UniProt ID: O00305
Chromosome Location: 2q22-q23
Pathway: rrhythmogenic right ventricular cardiomyopathy (ARVC);Axon guidance;Cardiac muscle contraction;Depolarization of the Presynaptic Terminal Triggers the Opening of Calcium Channels;Developmental Biology;Dilated cardiomyopathy;Hypertrophic cardiomyopathy (HCM)
Function: contributes_to high voltage-gated calcium channel activity;protein binding;contributes_to voltage-gated calcium channel activity;voltage-gated ion channel activity

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