Recombinant Human CA1 293 Cell Lysate

• Cat.No.: CA1-7917HCL

Specification

• Description: Antigen standard for carbonic anhydrase I (CA1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CA1 carbonic anhydrase I [ Homo sapiens ]
• Official Symbol: CA1
• Synonyms: CA1; carbonic anhydrase I; carbonic anhydrase 1; Car1; carbonic anhydrase B; carbonic dehydratase; carbonate dehydratase I; CAB; CA-I
• Gene ID: 759
• mRNA Refseq: NM_001128829
• Protein Refseq: NP_001122301
• MIM: 114800
• UniProt ID: P00915
• Chromosome Location: 8q21.2
• Pathway: C-MYB transcription factor network, organism-specific biosystem
• Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding

Reviews

05/19/2022

Swift protein purification, expedites our research timelines.

04/23/2021

Efficient analysis, aids our understanding of protein function.

09/17/2020

Reliable protein assays, critical for our scientific investigations.

Q&As

How does CA1 protein's phosphorylation change during synaptic stimulation?

CA1 protein's phosphorylation changes with synaptic stimulation, impacting its function.

What impact do post-translational modifications have on CA1 protein's subcellular localization and synaptic function?

Post-translational modifications can alter CA1 protein's subcellular localization and synaptic function.

How do epigenetic factors influence CA1 protein's function in memory formation?

Epigenetic factors influence CA1 protein's function in memory formation via modifications.

What is CA1 protein's specific role in regulating memory processes?

CA1 protein plays a specific role in memory processes, aiding memory formation.

What is CA1 protein's specific role in regulating synaptic plasticity?

CA1 protein regulates synaptic plasticity by modulating synaptic strength.

How do genetic factors regulate CA1 protein expression?

Genetic factors regulate CA1 protein expression, affecting its levels.

Can we develop a high-resolution imaging technique to monitor CA1 protein dynamics within dendritic spines?

Developing high-resolution imaging techniques for monitoring CA1 protein dynamics is feasible.