Recombinant Human C19orf62 293 Cell Lysate


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Recombinant Human C19orf62 293 Cell Lysate

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Cat.No. : BABAM1-8197HCL
Description : Antigen standard for chromosome 19 open reading frame 62 (C19orf62), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : BABAM1 BRISC and BRCA1 A complex member 1 [ Homo sapiens ]
Official Symbol : BABAM1
Synonyms : NBA1; HSPC142; MERIT40; C19orf62; BRISC and BRCA1-A complex member 1; BRCA1-A complex subunit MERIT40; new component of the BRCA1 A complex; new component of the BRCA1-A complex; new component of the BRCAA1 A complex; mediator of Rap80 interactions and targeting 40 kDa; mediator of RAP80 interactions and targeting subunit of 40 kDa
Gene ID : 29086
mRNA Refseq : NM_001033549
Protein Refseq : NP_001028721
MIM : 612766
UniProt ID : Q9NWV8
Chromosome Location : 19p13.11
Function : protein binding

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