Recombinant Human BRP44L 293 Cell Lysate

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Recombinant Human BRP44L 293 Cell Lysate

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Cat.No. : MPC1-8404HCL
Description : Antigen standard for brain protein 44-like (BRP44L) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : MPC1 mitochondrial pyruvate carrier 1 [ Homo sapiens ]
Official Symbol : MPC1
Synonyms : MPYCD; BRP44L; CGI-129; dJ68L15.3; mitochondrial pyruvate carrier 1; HSPC040 protein; brain protein 44-like protein
Gene ID : 51660
mRNA Refseq : NM_001270879
Protein Refseq : NP_001257808
MIM : 614738
Chromosome Location : 6q27
Pathway : Glycolysis and Gluconeogenesis, organism-specific biosystem; Pyruvate metabolism, organism-specific biosystem; The citric acid (TCA) cycle and respiratory electron transport, organism-specific biosystem
Function : molecular_function; pyruvate transmembrane transporter activity

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