"BLVRB" Related Products


Recombinant Human BLVRB 293 Cell Lysate

Cat.No.: BLVRB-8440HCL
Description: Antigen standard for biliverdin reductase B (flavin reductase (NADPH)) (BLVRB) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: BLVRB biliverdin reductase B (flavin reductase (NADPH)) [ Homo sapiens ]
Official Symbol: BLVRB
Synonyms: BLVRB; biliverdin reductase B (flavin reductase (NADPH)); Flavin reductase , FLR; flavin reductase (NADPH); SDR43U1; short chain dehydrogenase/reductase family 43U; member 1; FR; GHBP; BVR-B; NADPH-flavin reductase; NADPH-dependent diaphorase; green heme-binding protein; biliverdin-IX beta-reductase; short chain dehydrogenase/reductase family 43U, member 1; FLR; BVRB; MGC117413;
Gene ID: 645
mRNA Refseq: NM_000713
Protein Refseq: NP_000704
MIM: 600941
UniProt ID: P30043
Chromosome Location: 19q13.1-q13.2
Pathway: Heme degradation, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of porphyrins, organism-specific biosystem; Porphyrin and chlorophyll metabolism, organism-specific biosystem; Porphyrin and chlorophyll metabolism, conserved biosystem; Riboflavin metabolism, organism-specific biosystem; Riboflavin metabolism, conserved biosystem;
Function: biliverdin reductase activity; nucleotide binding; oxidoreductase activity; riboflavin reductase (NADPH) activity;

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