"BFSP2" Related Products


Recombinant Human BFSP2 293 Cell Lysate

Cat.No.: BFSP2-8461HCL
Description: Antigen standard for beaded filament structural protein 2, phakinin (BFSP2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: BFSP2 beaded filament structural protein 2, phakinin [ Homo sapiens ]
Official Symbol: BFSP2
Synonyms: CP47; CP49; LIFL-L; CTRCT12; PHAKOSIN; phakinin; 49 kDa cytoskeletal protein; beaded filament protein CP49; bfps2, Cytoskeletal protein, 49-kD; lens intermediate filament-like light; lens fiber cell beaded filament protein CP 47; lens fiber cell beaded filament protein CP 49
Gene ID: 8419
mRNA Refseq: NM_003571
Protein Refseq: NP_003562
MIM: 603212
UniProt ID: Q13515
Chromosome Location: 3q22.1
Function: structural constituent of cytoskeleton; structural constituent of eye lens

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