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Recombinant Human BCMO1 293 Cell Lysate

Cat.No.: BCMO1-8474HCL
Description: Antigen standard for beta-carotene 15,15-monooxygenase 1 (BCMO1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: BCMO1 beta-carotene 15,15-monooxygenase 1 [ Homo sapiens ]
Official Symbol: BCMO1
Synonyms: BCMO1; beta-carotene 15,15-monooxygenase 1; BCDO, BCDO1, beta carotene 15, 15 dioxygenase 1; beta,beta-carotene 15,15-monooxygenase; BCMO; FLJ10730; beta-carotene dioxygenase 1; beta-carotene 15, 15-dioxygenase 1; BCO; BCDO; BCO1; BCDO1;
Gene ID: 53630
mRNA Refseq: NM_017429
Protein Refseq: NP_059125
MIM: 605748
UniProt ID: Q9HAY6
Chromosome Location: 16q23.2
Pathway: Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Metabolism of steroid hormones and vitamins A and D, organism-specific biosystem; Retinol metabolism, organism-specific biosystem; Retinol metabolism, conserved biosystem; Vitamin A and carotenoid metabolism, organism-specific biosystem;
Function: beta-carotene 15,15-monooxygenase activity; beta-carotene 15,15-monooxygenase activity; metal ion binding; monooxygenase activity;

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