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Recombinant Human BCL2L1 293 Cell Lysate

Cat.No.: BCL2L1-8488HCL
Description: Antigen standard for BCL2-like 1 (BCL2L1), nuclear gene encoding mitochondrial protein, transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: BCL2L1 BCL2-like 1 [ Homo sapiens ]
Official Symbol: BCL2L1
Synonyms: BCL2L1; BCL2-like 1; bcl-2-like protein 1; Bcl X; bcl xL; bcl xS; BCL2L; BCLX; PPP1R52; protein phosphatase 1; regulatory subunit 52; apoptosis regulator Bcl-X; protein phosphatase 1, regulatory subunit 52; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; BCL-XL/S; DKFZp781P2092;
Gene ID: 598
mRNA Refseq: NM_138578
Protein Refseq: NP_612815
MIM: 600039
UniProt ID: Q07817
Chromosome Location: 20q11.21
Pathway: Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem; BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members, organism-specific biosystem;
Function: BH3 domain binding; identical protein binding; protein binding; protein heterodimerization activity; protein kinase binding;

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