Recombinant Human BCL10 293 Cell Lysate

• Cat.No.: BCL10-8490HCL

Specification

• Description: Antigen standard for B-cell CLL/lymphoma 10 (BCL10) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: BCL10 B-cell CLL/lymphoma 10 [ Homo sapiens ]
• Official Symbol: BCL10
• Synonyms: BCL10; B-cell CLL/lymphoma 10; B-cell lymphoma/leukemia 10; c E10; CARD containing molecule enhancing NF kB; CARD containing apoptotic signaling protein; CARD containing proapoptotic protein; CARD like apoptotic protein; CARMEN; caspase recruiting domain containing protein; CIPER; CLAP; mE10; hCLAP; cCARMEN; cellular-E10; cellular homolog of vCARMEN; CARD-containing proapoptotic protein; CARD containing molecule enhancing NF-kB; CARD-containing apoptotic signaling protein; caspase-recruiting domain-containing protein; CARD-containing molecule enhancing NF-kappa-B; mammalian CARD-containing adapter molecule E10; CED-3/ICH-1 prodomain homologous E10-like regulator; c-E10
• Gene ID: 8915
• mRNA Refseq: NM_003921
• Protein Refseq: NP_003912
• MIM: 603517
• UniProt ID: O95999
• Chromosome Location: 1p22
• Pathway: Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; BCR signaling pathway, organism-specific biosystem; Canonical NF-kappaB pathway, organism-specific biosystem
• Function: NF-kappaB binding; enzyme binding; contributes_to kinase activator activity; kinase binding; protease binding; protein C-terminus binding; protein binding; protein kinase B binding; protein kinase binding; protein self-association; transcription coactivator activity; transcription factor binding; ubiquitin binding; ubiquitin protein ligase binding