"BANF1" Related Products


Recombinant Human BANF1 293 Cell Lysate

Cat.No.: BANF1-8518HCL
Description: Antigen standard for barrier to autointegration factor 1 (BANF1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: BANF1 barrier to autointegration factor 1 [ Homo sapiens ]
Official Symbol: BANF1
Synonyms: BANF1; barrier to autointegration factor 1; barrier-to-autointegration factor; BAF; breakpoint cluster region protein 1; NGPS; BCRP1; D14S1460; MGC111161;
Gene ID: 8815
mRNA Refseq: NM_001143985
Protein Refseq: NP_001137457
MIM: 603811
UniProt ID: O75531
Chromosome Location: 11q13.1
Pathway: 2-LTR circle formation, organism-specific biosystem; APOBEC3G mediated resistance to HIV-1 infection, organism-specific biosystem; Autointegration results in viral DNA circles, organism-specific biosystem; Disease, organism-specific biosystem; Early Phase of HIV Life Cycle, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem;
Function: DNA binding;

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