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Recombinant Human B3GAT3 293 Cell Lysate

Cat.No.: B3GAT3-8545HCL
Description: Antigen standard for beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (B3GAT3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: B3GAT3 beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) [ Homo sapiens ]
Official Symbol: B3GAT3
Synonyms: B3GAT3; beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I); galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3; GlcAT I; glcUAT-I; Sqv-8-like protein; UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase; GLCATI;
Gene ID: 26229
mRNA Refseq: NM_012200
Protein Refseq: NP_036332
MIM: 606374
UniProt ID: O94766
Chromosome Location: 11q12
Pathway: Glycosaminoglycan biosynthesis - chondroitin sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - chondroitin sulfate, conserved biosystem; Glycosaminoglycan biosynthesis - heparan sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - heparan sulfate, conserved biosystem; Glycosaminoglycan biosynthesis, linkage tetrasaccharide, organism-specific biosystem; Glycosaminoglycan biosynthesis, linkage tetrasaccharide, conserved biosystem; Metabolic pathways, organism-specific biosystem;
Function: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity; manganese ion binding; metal ion binding; transferase activity;

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