Recombinant Human ATPIF1 293 Cell Lysate

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Recombinant Human ATPIF1 293 Cell Lysate

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Cat.No. : ATPIF1-8569HCL
Description : Antigen standard for ATPase inhibitory factor 1 (ATPIF1), nuclear gene encoding mitochondrial protein, transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ATPIF1 ATPase inhibitory factor 1 [ Homo sapiens ]
Official Symbol : ATPIF1
Synonyms : ATPIF1; ATPase inhibitory factor 1; ATPase inhibitor, mitochondrial; ATP synthase inhibitor protein; ATPase inhibitor protein; ATPI; ATPIP; IP; MGC1167; MGC8898; IF1; IF(1); inhibitor of F(1)F(o)-ATPase;
Gene ID : 93974
mRNA Refseq : NM_178191
Protein Refseq : NP_835498
UniProt ID : Q9UII2
Chromosome Location : 1p35.3
Pathway : Electron Transport Chain, organism-specific biosystem;
Function : ATPase binding; ATPase binding; ATPase inhibitor activity; ATPase inhibitor activity; angiostatin binding; calmodulin binding; enzyme inhibitor activity; protein homodimerization activity;

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