"ATP6V1B1" Related Products

Recombinant Human ATP6V1B1 293 Cell Lysate

Cat.No.: ATP6V1B1-8584HCL
Description: Antigen standard for ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 (ATP6V1B1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ATP6V1B1 ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 [ Homo sapiens ]
Official Symbol: ATP6V1B1
Synonyms: ATP6V1B1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1; ATP6B1, vacuolar proton pump 3 , VPP3; V-type proton ATPase subunit B, kidney isoform; Renal tubular acidosis with deafness; RTA1B; VATB; Vma2; V-ATPase B1 subunit; V-ATPase subunit B 1; vacuolar proton pump 3; H+-ATPase beta 1 subunit; vacuolar proton pump, subunit 3; vacuolar proton pump subunit B 1; endomembrane proton pump 58 kDa subunit; H(+)-transporting two-sector ATPase, 58kD subunit; VMA2; VPP3; ATP6B1; MGC32642;
Gene ID: 525
mRNA Refseq: NM_001692
Protein Refseq: NP_001683
MIM: 192132
UniProt ID: P15313
Chromosome Location: 2p13
Pathway: Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Epithelial cell signaling in Helicobacter pylori infection, organism-specific biosystem; Epithelial cell signaling in Helicobacter pylori infection, conserved biosystem; Insulin receptor recycling, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Metabolic pathways, organism-specific biosystem;
Function: ATP binding; hydrogen ion transmembrane transporter activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrolase activity; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; proton-transporting ATPase activity, rotational mechanism;

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