Recombinant Human ATP6V1B1 protein, GST-tagged

• Cat.No.: ATP6V1B1-1001H
• Product Overview: Human ATP6V1B1 full-length ORF ( AAH63411, 1 a.a. - 513 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq, Jul 2008]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 81.95 kDa
• AA Sequence: MAMEIDSRPGGLPGSSCNLGAAREH MQAVTRNYITHPRVTYRTVCSVNGP LVVLDRVKFAQYAEIVHFTLPDGTQ RSGQVLEVAGTKAIVQVFEGTSGID ARKTTCEFTGDILRTPVSEDMLGRV FNGSGKPIDKGPVVMAEDFLDINGQ PINPHSRIYPEEMIQTGISPIDVMN SIARGQKIPIFSAAGLPHNEIAAQI CRQAGLVKKSKAVLDYHDDNFAIVF AAMGVNMETARFFKSDFEQNGTMGN VCLFLNLANDPTIERIITPRLALTT AEFLAYQCEKHVLVILTDMSSYAEA LREVSAAREEVPGRRGFPGYMYTDL ATIYERAGRVEGRGGSITQIPILTM PNDDITHPIPDLTGFITEGQIYVDR QLHNRQIYPPINVLPSLSRLMKSAI GEGMTRKDHGDVSNQLYACYAIGKD VQAMKAVVGEEALTSEDLLYLEFLQ KFEKNFINQGPYENRSVFESLDLGW KLLRIFPKEMLKRIPQAVIDEFYSR EGALQDLAPDTAL
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ATP6V1B1 ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 [ Homo sapiens ]
• Official Symbol: ATP6V1B1
• Synonyms: ATP6V1B1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1; ATP6B1, vacuolar proton pump 3 , VPP3; V-type proton ATPase subunit B, kidney isoform; Renal tubular acidosis with deafness; RTA1B; VATB; Vma2; V-ATPase B1 subunit; V-ATPase subunit B 1; vacuolar proton pump 3; H+-ATPase beta 1 subunit; vacuolar proton pump, subunit 3; vacuolar proton pump subunit B 1; endomembrane proton pump 58 kDa subunit; H(+)-transporting two-sector ATPase, 58kD subunit; VMA2; VPP3; ATP6B1; MGC32642
• Gene ID: 525
• mRNA Refseq: NM_001692
• Protein Refseq: NP_001683
• MIM: 192132
• UniProt ID: P15313