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Recombinant Human ATP6V1A 293 Cell Lysate

Cat.No.: ATP6V1A-8585HCL
Description: Antigen standard for ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A (ATP6V1A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ATP6V1A ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A [ Homo sapiens ]
Official Symbol: ATP6V1A
Synonyms: ATP6V1A; ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A; ATP6A1, ATP6V1A1, ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha polypeptide, 70kD, isoform 1 , VPP2; V-type proton ATPase catalytic subunit A; VA68; Vma1; V-ATPase subunit A; V-ATPase A subunit 1; V-ATPase 69 kDa subunit 1; vacuolar ATPase isoform VA68; vacuolar proton pump subunit alpha; vacuolar proton pump alpha subunit 1; ATPase, H+ transporting, lysosomal, subunit A1; H(+)-transporting two-sector ATPase, subunit A; H+-transporting ATPase chain A, vacuolar (VA68 type); vacuolar ATP synthase catalytic subunit A, ubiquitous isoform; HO68; VPP2; ATP6A1; ATP6V1A1;
Gene ID: 523
mRNA Refseq: NM_001690
Protein Refseq: NP_001681
MIM: 607027
UniProt ID: P38606
Chromosome Location: 3q13.31
Pathway: Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Epithelial cell signaling in Helicobacter pylori infection, organism-specific biosystem; Epithelial cell signaling in Helicobacter pylori infection, conserved biosystem; Insulin receptor recycling, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Metabolic pathways, organism-specific biosystem;
Function: ATP binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrolase activity; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; nucleotide binding; proton-transporting ATPase activity, rotational mechanism;

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