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Recombinant Human ARSB 293 Cell Lysate

Cat.No. : ARSB-8677HCL
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Description : Antigen standard for arylsulfatase B (ARSB), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ARSB?arylsulfatase B [?Homo sapiens?(human) ]
Official Symbol : ARSB
Synonyms : ARSB; arylsulfatase B; ASB; G4S; MPS6; arylsulfatase B; N-acetylgalactosamine-4-sulfatase; NP_000037.2; EC 3.1.6.12; NP_942002.1
Gene ID : 411
mRNA Refseq : NM_000046
Protein Refseq : NP_000037
MIM : 611542
UniProt ID : P15848
Chromosome Location : 5q14.1
Pathway : CS/DS degradation, organism-specific biosystem; Chondroitin sulfate degradation, organism-specific biosystem; Disease, organism-specific biosystem
Function : N-acetylgalactosamine-4-sulfatase activity; arylsulfatase activity; metal ion binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Is the ARSB protein involved in any other biological processes or diseases? 12/22/2021

Although the primary function of the ARSB protein is related to the breakdown of GAGs, recent studies have suggested its involvement in other biological processes. It has been implicated in cellular signaling, inflammatory responses, and tissue remodeling. Additionally, ARSB mutations or dysregulation have been associated with certain cancers, neurodegenerative disorders, and metabolic disorders.

Are there any known mutations or variations in the ARSB gene? 01/22/2021

Yes, mutations in the ARSB gene can cause mucopolysaccharidosis type VI (MPS VI). Over 170 different mutations have been identified, including insertions, deletions, and point mutations, each leading to a deficiency in ARSB enzyme activity.

What are the consequences of ARSB protein deficiency? 10/14/2020

A deficiency in the ARSB protein leads to a rare inherited disorder called mucopolysaccharidosis type VI (MPS VI). This condition results in the accumulation of GAGs in various tissues and organs, causing various symptoms including skeletal abnormalities, cardiovascular issues, respiratory problems, and impaired vision and hearing.

How is the ARSB protein regulated in the body? 06/06/2020

The expression of the ARSB protein is regulated by various factors, including transcription factors and signaling pathways that control gene expression. Additionally, post-translational modifications such as phosphorylation or glycosylation can affect its activity and localization within the cell.

Are there any treatments available for mucopolysaccharidosis type VI? 02/24/2018

Yes, there are treatments available for mucopolysaccharidosis type VI. Enzyme replacement therapy (ERT) involves regular infusion of a man-made version of the ARSB enzyme to help break down the accumulated GAGs. Additionally, supportive therapies such as physical therapy, surgery, and medications are utilized to manage symptoms and complications. Gene therapy approaches are also being explored.

How is the ARSB protein related to lysosomal storage disorders? 09/01/2017

Mutations in the ARSB gene lead to a deficiency in the ARSB enzyme, resulting in the accumulation of GAGs within lysosomes. This accumulation is characteristic of lysosomal storage disorders, including mucopolysaccharidosis type VI. These disorders occur due to impaired lysosomal function, which leads to the build-up of undigested substances and subsequent cellular dysfunction.

Customer Reviews (3)

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01/25/2023

    the ARSB protein has proven to be a valuable asset in protein electron microscopy structure analysis.

    09/09/2019

      By utilizing the ARSB protein, researchers can unravel the intricate three-dimensional architecture of proteins, furthering our understanding of their function and facilitating the development of novel therapeutic interventions.

      08/03/2019

        The ARSB protein's superior performance in ELISA paves the way for robust and precise data analysis, providing invaluable insights into various biological processes.

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