Recombinant Human ARPP21 293 Cell Lysate

• Cat.No.: ARPP21-8680HCL

Specification

• Description: Antigen standard for cyclic AMP-regulated phosphoprotein, 21 kD (ARPP-21), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ARPP21 cAMP-regulated phosphoprotein, 21kDa [ Homo sapiens ]
• Official Symbol: ARPP21
• Synonyms: ARPP21; cAMP-regulated phosphoprotein, 21kDa; cAMP-regulated phosphoprotein 21; ARPP 21; R3H domain containing 3; R3HDM3; TARPP; thymocyte cAMP-regulated phosphoprotein; cyclic AMP-regulated phosphoprotein, 21 kD; RCS; ARPP-21; FLJ32997
• Gene ID: 10777
• mRNA Refseq: NM_001025068
• Protein Refseq: NP_001020239
• MIM: 605488
• UniProt ID: Q9UBL0
• Chromosome Location: 3p24.3
• Function: calmodulin binding; molecular_function; nucleic acid binding

Reviews

03/27/2023

Its high purity and stability ensure reliable and reproducible results, which are paramount for achieving meaningful scientific outcomes.

07/28/2017

The ARPP21 protein is renowned for its outstanding quality, making it an optimal choice to fulfill the requirements of my experimental investigations.

06/24/2016

Whether measuring analyte concentrations, screening for specific interactions, or monitoring immune responses, this protein's exceptional performance in ELISA experiments is truly commendable.

Q&As

Are there any drugs or compounds known to modulate ARPP21 activity?

Currently, there are no specific drugs or compounds known to directly modulate ARPP21 activity. Future research may identify potential compounds that could target ARPP21 for therapeutic purposes.

Is ARPP21 expressed in specific cell types or throughout the body?

ARPP21 is expressed in various cell types, including neurons in the brain. However, its expression pattern in different tissues and organs throughout the body is not well characterized.

Are there any diseases or disorders associated with ARPP21 dysfunction?

At this point, there is no specific information linking ARPP21 dysfunction to any particular diseases or disorders. However, further research is needed to fully understand its role and potential implications.

Can ARPP21 be used as a therapeutic target?

Currently, there is no direct evidence suggesting that ARPP21 can be targeted for therapeutic purposes. However, continued research may reveal its potential as a target for certain conditions in the future.

What are some research areas related to ARPP21?

Researchers are exploring the functions and mechanisms of ARPP21, its role in synaptic plasticity and cognitive processes, as well as its potential involvement in neurological disorders such as schizophrenia and addiction.

Are there any known interacting partners of ARPP21?

Yes, some studies suggest that ARPP21 interacts with other molecules or proteins involved in cellular signaling pathways, such as protein phosphatase 2A (PP2A), which is involved in regulating protein phosphorylation.

Where can I find more information about ARPP21?

To find more information about ARPP21 and its current research, you can refer to scientific journals, databases like PubMed, or academic websites specializing in neuroscience or protein research.